SBI/PrecisionX™ Gene Knock-out HR Targeting Vector (MCS1-EF1α-GFP-T2A-Puro-pA-MCS2)/10 µg/HR410PA-1-10 µg-免疫在线蚂蚁淘旗下平台

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商品详细SBI/PrecisionX™ Gene Knock-out HR Targeting Vector (MCS1-EF1α-GFP-T2A-Puro-pA-MCS2)/10 µg/HR410PA-1-10 µg

SBI/PrecisionX™ Gene Knock-out HR Targeting Vector (MCS1-EF1α-GFP-T2A-Puro-pA-MCS2)/10 µg/HR410PA-1-10 µg

商品编号： HR410PA-1-10 µg

品牌： System Biosciences

市场价： ¥18660.00

美元价： 11196.00

产地：美国(厂家直采)

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产品分类：其他

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商品介绍

Get precise genomic integration of your expression cassette

Use the PrecisionX™ Gene Knock-out HR Targeting Vector (MCS1-EF1α-GFP-T2A-Puro-pA-MCS2) to knock-out any gene or edit the genome. Clone your homology arms into MCS1 and MCS2, and use puromycin selection and GFP-positive imaging to find integrants. After you’ve identified clones with your gene-of-interest knocked-out or edited, you can remove the selection cassette using the Cre-LoxP system (learn more about Cre-LoxP excision here).

Why use an HR targeting vector?

Even though gene knock-outs can result from DSBs caused by Cas9 alone, SBI recommends the use of HR targeting vectors (also called HR donor vectors) for more efficient and precise mutation. HR donors can supply elements for positive or negative selection ensuring easier identification of successful mutation events. In addition, HR donors can include up to 6-8 kb of open reading frame for gene knock-ins or tagging, and, when small mutations are included in either 5’ or 3’ homology arms, can make specific, targeted gene edits.

Choose the right HR Targeting Vector for your project

Catalog #	HR Donor Vector	Features*	Application
			Gene Knock-out	Gene Knock-in	Gene Edits	Gene Tagging
HR100PA-1	MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4	Basic HR Donor
HR110PA-1	MCS1-EF1α-RFP-T2A-Puro-pA-MCS2	Removable RFP marker and puromycin selection
HR120PA-1	GFP-pA-LoxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCSPuro-pA-LoxP-MCS	Tag with GFP fusion Removable RFP marker and puromycin selection
HR130PA-1	T2A-GFP-pA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCSA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS	Co-express GFP with “tagged” gene via T2A Removable RFP marker and puromycin selection
HR150PA-1	GFP-T2A-Luc-pA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS	Tag with GFP fusion and co-express luciferase via T2A Removable RFP marker and puromycin selection
HR180PA-1	IRES-GFP-pA-loxP-MCS1-EF1α-RFP-T2A-Puro-pA-LoxP-MCS2	Co-express GFP with “tagged” gene via IRES Removable RFP marker and puromycin selection
HR210PA-1	MCS1-LoxP-EF1α-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2	Removable GFP marker, puromycin selection, and TK selection
HR220PA-1	GFP-pA-LoxP-EF1α-RFP-T2A-Hygro-pA-LoxP-MCS	Tag with GFP fusion Removable RFP ,arker and hygromycin Selection
HR410PA-1	MCS1-EF1α-GFP-T2A-Puro-pA-MCS2	Removable GFP marker and puromycin selection
HR510PA-1	MCS1-EF1α-RFP-T2A-Hygro-pA-MCS2	Removable RFP marker and hygromycin selection
HR700PA-1	MCS1-EF1α-GFP-T2A-Puro-pA-MCS2-PGK-hsvTK	Enrich for on-target integration with negative TK selection** Removable GFP marker and puromycin selection
HR710PA-1	MCS1-EF1α-RFP-T2A-Hygro-pA-MCS2-PGK-hsvTK	Enrich for on-target integration with negative TK selection** Removable RFP marker and hygromycin selection
HR720PA-1	MCS1-EF1α-Blasticidin-pA-MCS2-PGK-hsvTK	Enrich for on-target integration with negative TK selection** Removable blasticidin selection
GE602A-1	pAAVS1D-PGK-MCS-EF1α-copGFPpuro	First generation AAVS1-targeting HR Donor
GE603A-1	pAAVS1D-CMV-RFP-EF1α-copGFPpuro	First generation AAVS1-targeting HR Donor (positive control for GE602A-1)
GE620A-1	AAVS1-SA-puro-MCS	Second generation AAVS1-targeting HR Donor Promoterless to knock-in any gene or promoter-gene combination
GE622A-1	AAVS1-SA-puro-EF1α-MCS	Second generation AAVS1-targeting HR Donor Constitutive expression of your gene-of-interest
GE624A-1	AAVS1-SA-puro-MCS-GFP	Second generation AAVS1-targeting HR Donor Create reporter cell lines
CAS620A-1	AAVS1-SA-puro-EF1α-hspCas9	Knock-in Cas9 to the AAVS1 site
PBHR100A-1	MCS1-5"PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3" PB TR-MCS2	Use with the PiggyBac Transposon System Enables seamless gene editing with no residual footprint (i.e. completely remove vector sequences)
All HR Target Vectors except PBHR100A-1 contain LoxP sites. Any sequences that are integrated between the two LoxP sites can be removed through transient expression of Cre Recombinase. *The clever design of these HR Donors enables enrichment for on-target integration events. A PGK-hsvTK cassette is included outside of the homology arms. Because of this configuration, on-target integration that results from homologous recombination will not include the PGK-hsvTK cassette—only randomly-integrated off-target events will lead to integration of PGK-hsvTK and resulting TK activity. Therefore, TK selection will negatively select against off-target integrants. Click on any one of these vectors to see a diagram of how the negative selection works.

品牌介绍

美国SBI代理 System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒美国SBI代理 ExoQuick and ExoQuick-TC Products Product Size Catalog # ExoQuick serum exosome precipitation solution (5 ml) 75 reactions EXOQ5A-1 ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product 75 reactions EXOQ5TM-1 Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product 100 reactions TMEXO-1 ExoQuick serum exosome precipitation solution (20 ml) 300 reactions EXOQ20A-1 ExoQuick-TC for Tissue Culture Media and Urine (10ml) 10 reactions EXOTC10A-1 ExoQuick-TC for Tissue Culture Media and Urine (50ml) 50 reactions EXOTC50A-1 Exosome-depleted FBS Media Supplement - USA Certified 50 ml EXO-FBS-50A-1 Exosome-depleted FBS Media Supplement - USA Certified 250 ml EXO-FBS-250A-1 ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit 5 reactions EXOLP5A-1

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